By numerically simulating the whole process of construction using 3D finite element method, we analyzed some key technical issues of the ship lift tower structure of Three Gorges Project (TGP) in construction period. Such issues include the influences of construction schedule, wind load, embedded position of prestressed sleeves, and creep. According to simulation result, 1) the maximum tensile stress and deformation of tower structure poured in October were smaller than those in April, while the maximum vertical displacement in both cases occurred in the position where concrete was poured in high-temperature season. 2) Wind load had an evident influence on tower structure in the presence of a large height difference between the backfill elevation of wide groove and the casting elevation of inner wall, and before the top sealing of tower body. 3) The vertical displacement of rack and nut column, directly related to the embedded position of prestressed sleeves, was affected by the self-weight of the upper concrete, and such effect changed periodically with atmospheric temperature. 4) Creep had little effect on the rack and the nut column, and stayed within the range of installation accuracy requirements. The research findings offer reference for the construction schedule and accuracy control for the TGP ship lift tower.