为了探究微电流电解过程对铜绿微囊藻生长的影响,以铂钛作为阳极,碳黑聚四氟乙烯(C/PTFE)气体扩散电极为阴极,在微电流电解体系中可产生对铜绿微囊藻生长具有选择性抑制作用的活性物质H2O2。探究不同电解时间、电流密度和气体流量对藻细胞生长的影响,结果表明:微电流电解的最佳条件为100 mL细胞密度为5×105个/mL 的藻液在10 mA/cm2的电流密度下以0.4 L/min的气体流量电解60 min。藻细胞光密度OD680从0.035降至0.003,表明藻细胞的生长完全受到抑制。测定电解处理前后藻细胞的叶绿素荧光参数Fv/Fm、Y(Ⅱ)、Y(NO)等可知,电解处理后藻细胞的光合作用机制已遭到完全破坏。测定电解过程中产生H2O2的质量浓度为79 mg/L,并且经过6次循环实验后,C/PTFE气体扩散电极产生H2O2质量浓度仍为首次使用产生H2O2质量浓度的66%(52 mg/L),说明C/PTFE气体扩散电极的稳定性良好,具有较大的应用前景。该研究可为微电流电解抑制蓝藻水华提供新手段。
Abstract
Platinum titanium served as the anode, while a carbon black polytetrafluoroethylene (C/PTFE) gas diffusion electrode was utilized as the cathode in order to facilitate the production of H2O2 through low-amperage electrolysis, with the aim of inhibiting the growth of Microcystis aeruginosa. Through a series of experimental investigations involving varying electrolysis time, current density, and gas flow, the optimal conditions for inhibiting Microcystis aeruginosa were determined. Specifically, the optimal configuration involved the electrolysis of 100 mL of 5×105 cells/mL algae solution at a current density of 10 mA/cm2 and a gas flow rate of 0.4 L/min for a duration of 60 minutes. Following electrolysis, the optical density (OD680) of the algae cells decreased from 0.035 to 0.003, indicating the complete inhibition of algae cell growth. Additionally, the measurement of chlorophyll fluorescence parameters, such as Fv/Fm, Y(Ⅱ), and Y(NO), demonstrated the substantial disruption to the photosynthetic mechanism of the algae, further indicating the complete decay of the algae population. The concentration of H2O2 generated during electrolysis was determined to be 79 mg/L. Furthermore, even after six cycles of reuse, the C/PTFE cathode maintained 66% (52 mg/L) of the initial H2O2 concentration, highlighting the excellent stability and promising application potential of the C/PTFE electrode. This study presents a novel approach to effectively inhibit cyanobacterial blooms through low-amperage electrolysis, offering a new avenue for remediation.
关键词
气体扩散电极 /
微电流 /
过氧化氢 /
铜绿微囊藻 /
抑制作用
Key words
gas diffusion electrode /
low-amperage electric current /
hydrogen peroxide /
microcystis aeruginosa /
inhibiting effect
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基金
武汉市应用基础前沿项目(2020020601012285);中央级公益性科研院所基本科研业务费项目(CKSF2021480/SH, CKSF2017062/SH)
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