This research is made in accordance with the No.1 Central Document Policy and the most stringent water resources management planning issued by the Ministry of Water Resources. The status quo and planning of water use in the main stream and tributary of Yangtze river basin and provincial administrative regions are investigated on the basis of national water resources comprehensive planning and the hydrological regime in the basin. The targets of controlling the water use total amount for the whole basin are divided and implemented in each provincial administrative region and the main tributaries, and are ultimately allocated to each specific control section. The research result could serve as quantification technological support and decision-making basis for organizations in the basin to carry out the No.1 Central Document Policy and to establish the most stringent water resources management system combining total amount control with quota management.