Influencing Factors and Mechanism of Giardia Inactivationin Water by Ultrasonic Irradiation

RAN Zhi-lin; LI Jin-yu

doi:10.3969/j.issn.1001-5485.2014.08.0062014,31(08):29-34

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Journal of Changjiang River Scientific Research Institute ›› 2014, Vol. 31 ›› Issue (8) : 29-34. DOI: 10.3969/j.issn.1001-5485.2014.08.0062014,31(08):29-34

RIVER-LAKE SEDIMENTATION AND REGULATION

Influencing Factors and Mechanism of Giardia Inactivationin Water by Ultrasonic Irradiation

RAN Zhi-lin¹, LI Jin-yu²

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Abstract

Ultrasonic was adopted for inactivating Giardia in water. The effect of organic substance, turbidity and inorganic ions on ultrasonic inactivation was investigated. The mechanism of ultrasonic inactivation of Giardia was studied by scanning electron microscopy. Results indicated that the inactivation rate decreased with the increase of humic acid concentration, when the concentration of humic acid was 50.0 mg/L, the inactivation rate reduced to 80.6%. In the range of 0-20 NTU turbidity, the inactivation rate first increased and then decreased, when the turbidity was 1.0 NTU, the inactivation rate reached maximum (98.6%). The effect of Ca²⁺, Zn²⁺ and SO^2-₄ content on the inactivation was little, and low concentration of Ca²⁺(1.0 mg/L) could increase the inactivation rate to 96.3%. The increase of HCO^-₃ concentration could reduce the inactivation rate, when the concentration was 100 mg/L the inactivation rate was 83.8%. It was obvious that ultrasonic damaged the cell structure of Giardia by scanning electron microscopy morphological observation, which suggested that the mechanical destruction due to the cavitation was the major factor of inactivating Giardia by ultrasonic.

Key words

ultrasonic inactivation / Giardia / inactivation rate / scanning electron microscopy

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RAN Zhi-lin, LI Jin-yu. Influencing Factors and Mechanism of Giardia Inactivationin Water by Ultrasonic Irradiation[J]. Journal of Changjiang River Scientific Research Institute. 2014, 31(8): 29-34 https://doi.org/10.3969/j.issn.1001-5485.2014.08.0062014,31(08):29-34

References

[1] CUI F Y, ZUO J L, ZHAO Z W, et al. Review of Giardia and Cryptosporidium in Drinking Water[J]. Harbin Institute Technology, 2006, 38(9): 1487-1491.
[2] BALDURSSON S, KARANIS P. Waterborne Transmission of Protozoan Parasites: Review of Worldwide Outbreaks-An Update 2004-2010[J]. Water Research, 2011, 45(20): 6603-6614.
[3] 邓明俊, 肖西志. 蓝氏贾第鞭毛虫检测技术研究进展[J]. 动物医学进展, 2012, 33(12): 168-173. (DENG Ming-jun, XIAO Xi-zhi. Progress on Detection Methods for Giardia lamblia[J]. Progress in Veterinary Medicine, 2012, 33(12): 168-173. (in Chinese))
[4] GB 5749—2006,生活饮用水卫生标准[S]. 北京: 中国标准出版社, 2006. (GB 5749—2006, Standard of Drinking Water[S]. Beijing: Chinese Standard Press, 2006. (in Chinese))
[5] WICKRAMANAYAKE G B, ALAN J R, SPROUL O J. Inactivation of Giardialamblia cysts with Ozone[J]. Applied and Environmental Microbiology, 1984, 48(3): 671-672.
[6] EUGENE W R, JOHN C H. Inactivation of Giardia cysts by Ultraviolet Irradiation[J]. Applied and Environmental Microbiology, 1981, 42(3): 546-547.
[7] HAYES S L, RICE E W, WARE M W, et al. Low Pressure Ultraviolet Studies for Inactivation of Giardiamuris cysts[J]. Journal of Applied Microbiology, 2003, 94(1): 54-59.
[8] 李金玉,冉治霖. H₂O₂/O₃协同灭活水中隐孢子虫影响因素研究[J]. 长江科学院院报,2013, 30(9): 17-21.(LI Jin-yu, RAN Zhi-lin. Synergistic Effect of H₂O₂/O₃ on the Inactivation of Cryptosporidium parvum in Water[J]. Journal of Yangtze River Scientific Research Institute, 2013, 30(9): 17-21. (in Chinese))
[9] MONTEMAYOR M, GALOFRE B, RIBAS F, et al. Comparative Study between Two Laser Scanning Cytometers and Epifluorescence Microscopy for the Detection of Cryptosporidium oocysts in Water [J]. Cytometry Part A, 2007,71(3):163-169.
[10]王琳, 王宝贞. 饮用水深度处理技术[M]. 北京:化学工业出版社, 2001: 276-338.(WANG Lin, WANG Bao-zhen. Advanced Treatment of Drinking Water[M]. Beijing: Chemical Industry Press, 2001: 276-338. (in Chinese))
[11]HUA I, THOMPSON J E. Inactivation of Escherichia coli by Sonication at Discrete Ultrasonic Frequencies[J]. Water Research, 2002, 34(10): 3888-3893.
[12]靳慧霞, 董滨, 孙颖, 等. 超声协同紫外灭活大肠杆菌试验研究[J]. 环境科学与技术,2010,33(11):22-27.(JIN Hui-xia, DONG Bin, SUN Ying, et al. Synergistic Efficiency of E. Coli Inactivation under US and UV[J]. Environmental Science & Technology, 2010,33(11):22-27. (in Chinese))
[13]闫坤, 吕加平, 刘鹭, 等. 超声波对液态奶中枯草芽孢杆菌的杀菌作用[J]. 中国乳品工业, 2010, 38(2): 4-6.(YAN Kun,LV Jia-ping,LIU Lu, et al. Sterilization of Ultrasound on liquid milk in Bacillus subtilis[J]. China Dairy Industry, 2010, 38(2): 4-6. (in Chinese))
[14]李绍峰, 冉治霖, 高云霞, 等. 隐孢子虫3种检测方法比较[J]. 环境工程学报, 2009, 3(1): 47-51. (LI Shao-feng, RAN Zhi-lin, GAO Yun-xia, et al. Comparison of Three Methods for Detection of Cryptosporidium[J]. Chinese Journal of Environmental Engineering, 2009, 3(1): 47-51. (in Chinese))
[15]冉治霖, 李绍峰, 朱静, 等. 二氧化氯灭活水中隐孢子虫的影响因素及机理研究[J].中国环境科学, 2011, 31(6): 904-909.(RAN Zhi-lin, LI Shao-feng, ZHU Jing, et al. Cryptosporidium Inactivated by Chlorine Dioxide in Water and Disinfect Mechanisms[J]. China Environmental Science, 2011, 31(6): 904-909. (in Chinese))
[16]冉治霖, 李绍峰, 黄君礼, 等. 氯气灭活饮用水中隐孢子虫的影响因素[J]. 中国环境科学, 2011, 30(6): 786-790.(RAN Zhi-lin, LI Shao-feng, HUANG Jun-li, et al. Effect of Various Factors on Chlorine Inactivating Cryptosporidium in Water[J]. China Environmental Science, 2011, 30(6): 786-790. (in Chinese))
[17]胡健龙, 冉治霖, 李绍峰, 等. 超声灭活饮用水中隐孢子虫研究[J]. 中国环境科学, 2014, 34(3): 431-436. (HU Jian-long, RAN Zhi-lin, LI Shao-feng, et al. Inactivation of Cryptosporidium Parvum in Drinking Water by Ultrasonic Irradiation[J]. China Environmental Science, 2014, 34(3): 431-436. (in Chinese))
[18]HAYAKAWA M, NONOMURA H. Humic Acid-vitamin Agar, a New Medium for the Selective Isolation of Soil Actinomycetes[J]. Journal of Fermentation Technology, 1987, 65(5): 501-509.
[19]HOIGN J, BADER H. Rate Constants of Reactions of Ozone with Organic and Inorganic Compounds in Water Ⅲ: Inorganic Compounds and Radicals [J].Water Research, 1985, 19(8): 993-1004.
[20]NADDEO V, BELGIORNO V, NAPOLI R M A. Behaviour of Natural Organic Matter During Ultrasonic Irradiation[J]. Desalination, 2007, 210(1/3):175.
[21]SRAVAN K S, SHYAM S P, GOGATE P R, et al.Characterization of Sonochemical Reactor for Physicochemical Transformations[J]. Industrial ＆ Engineering Chemistry Research, 2009, 48(21): 9402-9407.
[22]TORU T, KYUICHI Y, MANICKAM S, et al. Correlation between Acoustic Cavitation Noise and Yield Enhancement of Sonochemical Reaction by Particle Addition[J]. Journal of Physical Chemistry A, 2005, 109(21) : 4869-4872.
[23]JASON W R, JACK S, GELI G, et al. Calcium Efflux is Essential for Bacterial Survival in the Eukaryotic Host[J]. Molecular Microbiology, 2008, 70(2): 435-444.
[24]GOEL M, HONGQIANG H, MUJUMDAR A S, et al. Sonochemical Decomposition of Volatile and Non-volatile Organic Compounds: A Comparative Study[J]. Water Research, 2004, 38:4247-4261.
[25]IKUKO O, TOMO T, YASUNORI O, et al. Comparison between the Effects of Ultrasound and γ-rays on the Inactivation of Saccharomyces cerevisiae: Analyses of Cell Membrane Permeability and DNA or RNA Synthesis by Flow Cytometry[J]. Ultrasonics Sonochemistry,2009,16:532-536.
[26]CUI X, JEFFREY T W, LIU G, et al. Effects of Primary Sludge Particulate(PSP) Entrapment on Ultrasonic(20kHz) Disinfection of Escherichia Coli[J]. Water Research, 2011,45(11): 3300-3308.