Sluice is a large-volume concrete structure with thin walls. Practice have shown that concrete sluice structure constructed in low-temperature season is subjected to surface cracks in the early stage of pouring if no temperature control measure is taken, and such surface cracks would penetrate through in later stage. To clarify the influence of temperature control measures on the temperature field, stress field and cracking risk of the sluice is the prerequisite of determining temperature control indices and formulating anti-cracking measures. With a sluice project under construction in low-temperature season as a case study, we examined the spatial and temporal characteristics of temperature field and stress field by simulating the construction process using 3D finite element method. On this basis, we analyzed the sensitivities of stress to pouring temperature, surface temperature preservation, and water cooling. Results manifested that single measure has limited effect. Multiple measures including temperature-control pouring, surface temperature preservation, and water cooling must be coordinated to effectively control the temperature stress of the sluice and avoid cracks.